pGEX-5x3-hMeCP2-delN256-R270X

48089 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of GST tagged AT-Hook2-R270X
Depositor
Huda Zoghbi
Article
Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
Insert
Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
Tags
GST
Mutation
deleted the N-terminal 256 codons, expresses AT-H…
Available Since
April 17, 2014
Availability
Available

Sequence Information

Length
943 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
Factor Xa site Factor Xa recognition and cleavage site 9..21 Fwd
DNA Sequence
ATCGAAGGTCGT
Protein Sequence
IEGR
7xHis 6xHis affinity tag 366..387 Fwd
DNA Sequence
CACCACCACCATCACCACCAC
Protein Sequence
HHHHHHH
Myc Myc (human c-Myc proto-oncogene) epitop… 732..762 Fwd
DNA Sequence
GAACAGAAACTGATCTCTGAAGAAGACCTG
Protein Sequence
EQKLISEEDL
6xHis 6xHis affinity tag 762..780 Fwd
DNA Sequence
CATCATCACCATCACCAC
Protein Sequence
HHHHHH