pET22b-EC12WTC9AAvi

47925 Mus musculus Bacterial Expression

Plasmid Information

Purpose
Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.
Depositor
Tanja Kortemme
Article
Ritterson et al J Am Chem Soc. 2013 Aug 7.
Insert
E-Cadherin EC12
Tags
6xHis, Avi Tag, and TEV Cleavage
Mutation
changed Cysteine 9 to Alanine
Promoter
T7
Available Since
Sept. 27, 2013
Availability
Available

Sequence Information

Length
932 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 133..151 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
AviTag(TM) — peptide tag that allows for enzymatic b… 832..877 Fwd
DNA Sequence
GGTCTGAACGACATCTTCGAGGCTCAGAAAATCGAATGGCACGAA
Protein Sequence
GLNDIFEAQKIEWHE
6xHis — 6xHis affinity tag 886..904 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 42..61 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 61..86 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC