pET22b-EC12WTC9A — PlasmidDB

Plasmid Information

Purpose

Expresses E-cadherin EC12 WT with C9A mutation in E.Coli.

Depositor

Tanja Kortemme

Article

Ritterson et al J Am Chem Soc. 2013 Aug 7.

Insert

E-Cadherin EC12

Length

939 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	135..153	Fwd
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH
6xHis	—	6xHis affinity tag	837..855	Fwd
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	44..63	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	63..88	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC