pFAB1935

47860 Synthetic Bacterial Expression

Plasmid Information

Purpose
BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency
Depositor
Drew Endy
Article
Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:
Insert
his_T_min_linker_crp_T_min double terminator
Mutation
concatenate of his_min and crp_min
Promoter
pLTetO15
Available Since
Dec. 3, 2013
Availability
Available

Sequence Information

Length
993 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 105..200 Rev
DNA Sequence
GACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAAT