pFAB806 — PlasmidDB

Plasmid Information

Purpose

BIOFAB reporter plasmid for measuring lambda tR2 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

lambda tR2 terminator

Promoter

pLTetO4

Available Since

Dec. 3, 2013

Availability

Available

Length

987 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
PLtetO-1 promoter	—	—	373..447	Fwd
DNA Sequence TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCACATCAGCAGGACGCACTGACC

protein_bind 2

Label	Gene	Product	Position	Strand
tet operator	tetO	—	373..392	Fwd
DNA Sequence TCCCTATCAGTGATAGAGA
tet operator	tetO	—	398..417	Fwd
DNA Sequence TCCCTATCAGTGATAGAGA