pDR-meptrac-H194E

47764 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
expresses MepTrac-H194E in yeast
Depositor
Wolf Frommer
Article
De Michele et al eLife. 2013 Jul 2;2:e00800.
Insert
MepTrac-H194E
Tags
mcpGFP
Mutation
H194E
Promoter
PMA
Available Since
Sept. 16, 2013
Availability
Available

Sequence Information

Length
1,005 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
VC155 — C-terminal fragment of mVenus for use i… 35..287 Rev
DNA Sequence
GACAAGCAGAAGAACGGCATCAAGGCGAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCTACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCGTCCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG
Protein Sequence
DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
protein_bind 1
Label Gene Product Position Strand
attB1 mutant version of attB — 964..989 Rev
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT