pmalc4x_G252R_TEV

47704 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation G252R
Depositor
Raquel Lieberman
Article
Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
Insert
Olfactomedin-like domain with G252R mutation (MYOC Human)
Tags
MBP
Mutation
changed Glycine 252 Arginine
Promoter
Ptac
Available Since
Sept. 9, 2013
Availability
Available

Sequence Information

Length
1,099 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
TEV site tobacco etch virus (TEV) protease recog… 4..25 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGAGC
Protein Sequence
ENLYFQS