pmalc4x_I499F_TEV

47703 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F
Depositor
Raquel Lieberman
Article
Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
Insert
Olfactomedin-like domain with I499F mutation (MYOC Human)
Tags
MBP
Mutation
changed I499F
Promoter
Ptac
Available Since
Sept. 9, 2013
Availability
Available

Sequence Information

Length
1,099 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
TEV site tobacco etch virus (TEV) protease recog… 4..25 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGAGC
Protein Sequence
ENLYFQS