pmalc4x_K398R_TEV

47701 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398R
Depositor
Raquel Lieberman
Article
Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
Insert
Olfactomedin-like domain with K398R mutation (MYOC Human)
Tags
MBP
Mutation
changed Lysine 398 to Arginine
Promoter
Ptac
Available Since
Sept. 9, 2013
Availability
Available

Sequence Information

Length
1,099 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
TEV site tobacco etch virus (TEV) protease recog… 4..25 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGAGC
Protein Sequence
ENLYFQS