pmalc4x_E323K_TEV

47699 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323K
Depositor
Raquel Lieberman
Article
Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
Insert
Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
Tags
MBP
Mutation
changed glutamic acid 323 to lysine
Promoter
Ptac
Available Since
Sept. 9, 2013
Availability
Available

Sequence Information

Length
1,099 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
TEV site tobacco etch virus (TEV) protease recog… 4..25 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGAGC
Protein Sequence
ENLYFQS