Cyprinodon variegatus cryaa

Plasmid Information

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Promoter

Available Since

Sept. 27, 2013

Availability

Available

Length

922 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	553..571	Fwd
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	637..685	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG