Danio rerio cryaa V62T

47630 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Recombinant protein production
Depositor
Mason Posner
Article
Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371
Insert
Alpha A-crystallin (cryaa Zebrafish)
Mutation
Changed Valine 62 to Threonine
Promoter
T7
Available Since
Oct. 21, 2013
Availability
Available

Sequence Information

Length
781 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 589..607 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 673..721 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG