Danio rerio cryaa T147V

47628 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Recombinant protein production
Depositor
Mason Posner
Article
Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371
Insert
Alpha A-crystallin (cryaa Zebrafish)
Mutation
Changed Theronine 147 to Valine
Promoter
T7
Available Since
Sept. 30, 2013
Availability
Available

Sequence Information

Length
830 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 582..600 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 666..714 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG