pGEX-4T-1 3xHA-MKK6-EE

47579 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6
Depositor
Kevin Janes
Article
Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-
Insert
MKK6-EE (MAP2K6 Human)
Tags
3xHA and GST
Mutation
Ser207 to Glu, Thr211 to Glu
Available Since
Aug. 28, 2013
Availability
Available

Sequence Information

Length
776 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
3xHA three tandem HA epitope tags 13..94 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCTTACCCATACGATGTTCCAGATTACGCTTACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYAYPYDVPDYAYPYDVPDYA