pGEX-4T-1 3xHA-MKK4-EE

47577 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4
Depositor
Kevin Janes
Article
Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-
Insert
MKK4-EE (MAP2K4 Human)
Tags
3xHA and GST
Mutation
Lacks the first 36 residues of the RefSeq protein…
Available Since
Aug. 28, 2013
Availability
Available

Sequence Information

Length
926 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
3xHA — three tandem HA epitope tags 13..94 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCTTACCCATACGATGTTCCAGATTACGCTTACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYAYPYDVPDYAYPYDVPDYA