pGEX-4T-1 3xHA-MEK1-DD

47576 Mus musculus Bacterial Expression

Plasmid Information

Purpose
Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1
Depositor
Kevin Janes
Article
Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-
Insert
MEK1-DD (Map2k1 Mouse)
Tags
3xHA and GST
Mutation
Ser218 to Asp, Ser221 to Asp
Available Since
Aug. 28, 2013
Availability
Available

Sequence Information

Length
937 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
3xHA — three tandem HA epitope tags 13..94 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCTTACCCATACGATGTTCCAGATTACGCTTACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYAYPYDVPDYAYPYDVPDYA