pET12a-AL-09 Y32F

47082 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Marina Ramirez-Alvarado
Article
DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8
Insert
AL-09 Y32F (IGKV3-20 Human)
Tags
ompT
Mutation
Y32F
Promoter
T7
Available Since
Aug. 1, 2013
Availability
Available

Sequence Information

Length
914 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
T7 promoter — — 51..70 Fwd
DNA Sequence
TAATACGACTCACTATAGG
terminator 1
Label Gene Product Position Strand
T7 terminator — — 588..636 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG