pSNR52-sgTET

46923 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter
Depositor
Stanley Qi, Jonathan Weissman
Article
Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
Insert
sgRNA targeting endogenous TRE elements of pTET07 promoter
Promoter
SNR52
Available Since
Oct. 1, 2013
Availability
Available

Sequence Information

Length
950 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 3
Label Gene Product Position Strand
SNR52 promoter — — 39..308 Fwd
DNA Sequence
TCTTTGAAAAGATAATGTATGATTATGCTTTCACTCATATTTATACAGAAACTTGATGTTTTCTTTCGAGTATATACAAGGTGATTACATGTACGTTTGAAGTACAACTCTAGATTTTGTAGTGCCCTCTTGGGCTAGCGGTAAAGGTGCGCATTTTTTCACACCCTACAATGTTCTGTTCAAAAGATTTTGGTCAAACGCTGTAGAAGTGAAAGTTGGTGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATC
T3 promoter — — 455..474 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter — — 542..573 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 518..535 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 587..609 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
terminator 1
Label Gene Product Position Strand
SUP4 terminator — — 407..427 Fwd
DNA Sequence
TTTTTTTGTTTTTTATGTCT