pAC4 Frk-SH2

46428 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Bruce Mayer
Article
Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.
Insert
Frk (FRK Human)
Tags
AviTag, GST, and Thrombin site
Mutation
contains amino acids 108-213
Promoter
Trc
Available Since
July 10, 2013
Availability
Available

Sequence Information

Length
742 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 6..24 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
AviTag(TM) peptide tag that allows for enzymatic b… 360..405 Fwd
DNA Sequence
GGTCTGAACGACATCTTCGAGGCTCAGAAAATCGAATGGCACGAA
Protein Sequence
GLNDIFEAQKIEWHE
terminator 1
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB 624..711 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT