pGEX-4T-1-p19-T7-PLK1

46312 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Judy Lieberman
Article
Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do
Tags
6XHis and GST
Available Since
Jan. 2, 2014
Availability
Available

Sequence Information

Length
590 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 9..27 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
6xHis 6xHis affinity tag 543..561 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter 567..586 Fwd
DNA Sequence
TAATACGACTCACTATAGG