pGEX-4T-1-p19-T7-EGFPFL

46310 Synthetic Bacterial Expression

Plasmid Information

Depositor
Judy Lieberman
Article
Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do
Tags
6XHis and GST
Available Since
Jan. 2, 2014
Availability
Available

Sequence Information

Length
606 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 12..30 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
6xHis 6xHis affinity tag 546..564 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter 570..589 Fwd
DNA Sequence
TAATACGACTCACTATAGG