Danio rerio cryaa

46166 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Recombinant protein production
Depositor
Mason Posner
Article
Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371
Insert
Alpha A-crystallin (cryaa Zebrafish)
Promoter
T7
Available Since
Oct. 7, 2013
Availability
Available

Sequence Information

Length
798 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 584..602 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 668..716 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG