pCVL.Active/Repressed TLR (Sce)

45575 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease
Depositor
Andrew Scharenberg
Article
Kuhar et al Nucleic Acids Res. 2013 Oct 10.
Tags
T2A dislinker and iRFP
Mutation
embedded I-Sce I TS from 163-185
Promoter
SFFV
Available Since
Nov. 4, 2013
Availability
Available

Sequence Information

Length
914 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
T2A 2A peptide from Thosea asigna virus cap… 125..179 Rev
DNA Sequence
GAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCGGGCCCT
Protein Sequence
EGRGSLLTCGDVEENPGP