PL2x2_5

45491 Synthetic Bacterial Expression

Plasmid Information

Depositor

David Baker

Insert

PL2x2_5

Tags

6xHis

Mutation

changed E30Y of Di-IV_5_PL2x2_6

Available Since

May 23, 2013

Availability

Available

Sequence Information

Length

855 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	473..491	Fwd
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	67..86	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	86..111	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	557..605	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG