Di-V_7_R3x1_7

45488 Synthetic Bacterial Expression

Plasmid Information

Depositor
David Baker
Article
Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi:
Insert
Di-V_7_R3x1_7
Tags
6xHis
Available Since
May 23, 2013
Availability
Available

Sequence Information

Length
873 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
6xHis 6xHis affinity tag 458..476 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter 58..77 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator 77..102 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
terminator 1
Label Gene Product Position Strand
T7 terminator 542..590 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG