Di-II_10_R2x2_769 — PlasmidDB

Plasmid Information

Depositor

David Baker

Article

Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi:

Insert

Di-II_10_R2x2_769

Length

753 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	463..481	Fwd
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	63..82	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	82..107	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	547..595	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG