Di-I_5_FR28 — PlasmidDB

Plasmid Information

Depositor

David Baker

Article

Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi:

Insert

Di-I_5_FR28

Length

791 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	389..407	Fwd
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	58..77	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	77..102	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	473..521	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG