pET-15b PAI-RBP

45390 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Thomas Gelehrter
Article
Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E
Insert
PAI-RBP (SERBP1 Human)
Tags
His
Available Since
June 20, 2013
Availability
Available

Sequence Information

Length
840 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 148..166 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 175..193 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 49..68 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 68..93 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC