pETCON-DnvLB16 M40R;W41H;H42S

45125 Synthetic Yeast Expression

Plasmid Information

Depositor
David Baker
Article
Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.
Insert
DnvLB16 M40R;W41H;H42S
Tags
Aga2p for yeast surface display and c-myc epitope…
Mutation
M40R;W41H;H42S to DnvLB16
Promoter
GAL
Available Since
July 8, 2013
Availability
Available

Sequence Information

Length
887 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 352..382 Rev
DNA Sequence
GAACAAAAGCTTATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
HA — HA (human influenza hemagglutinin) epit… 826..853 Rev
DNA Sequence
TACCCATACGACGTTCCAGACTACGCT
Protein Sequence
YPYDVPDYA
Factor Xa site — Factor Xa recognition and cleavage site 853..865 Rev
DNA Sequence
ATTGAAGGTAGA
Protein Sequence
IEGR
promoter 1
Label Gene Product Position Strand
T7 promoter — — 4..23 Fwd
DNA Sequence
TAATACGACTCACTATAGG