pET29b-HtsptLB12v.3

45123 Synthetic Bacterial Expression

Plasmid Information

Depositor
David Baker
Article
Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.
Insert
HtsptLB12v.3
Tags
6xHis
Promoter
T7
Available Since
July 8, 2013
Availability
Available

Sequence Information

Length
914 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 644..662 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 67..86 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 86..111 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
terminator 1
Label Gene Product Position Strand
T7 terminator — — 728..776 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG