pET29b-HtsptLB12

45122 Synthetic Bacterial Expression

Plasmid Information

Depositor
David Baker
Article
Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.
Insert
HtsptLB12
Tags
6xHis
Promoter
T7
Available Since
July 5, 2013
Availability
Available

Sequence Information

Length
856 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 632..650 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 55..74 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 74..99 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
terminator 1
Label Gene Product Position Strand
T7 terminator — — 716..764 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG