| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 150..168 | Fwd | |
|
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
|
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| thrombin site | — | thrombin recognition and cleavage site | 177..195 | Fwd | |
|
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 51..70 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 70..95 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
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