p107.1

44517 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor

Michael Grunstein

Article

Hecht et al Cell. 1995 Feb 24;80(4):583-92.

Insert

Histone 4 N-terminus aa1-34

Tags

GST

Mutation

aa1-34 of H4

Available Since

May 21, 2013

Availability

Available

Sequence Information

Length

882 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 2

Label	Gene	Product	Position	Strand
thrombin site	—	thrombin recognition and cleavage site	121..139	Rev
DNA Sequence CTGGTTCCGCGTGGATCC Protein Sequence LVPRGS
GST	—	glutathione S-transferase from Schistos…	145..799	Rev
DNA Sequence ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAA Protein Sequence MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK

promoter 1

Label	Gene	Product	Position	Strand
tac promoter	—	—	845..874	Rev
DNA Sequence TTGACAATTAATCATCGGCTCGTATAATG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	821..838	Fwd
DNA Sequence TTGTTATCCGCTCACAA