Pet28a_Synthetic_Human_H2A.1

42634 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Joe Landry
Insert
Human H2A.1 (H2AC13 Human, Synthetic)
Tags
Untagged
Mutation
Codon Optimized
Promoter
T7
Available Since
Feb. 25, 2013
Availability
Available

Sequence Information

Length
1,028 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 34..52 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 2
Label Gene Product Position Strand
T7 promoter — — 531..550 Rev
DNA Sequence
TAATACGACTCACTATAGG
lacI promoter lacI — 858..936 Fwd
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
protein_bind 1
Label Gene Product Position Strand
lac operator — — 506..531 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC