Pet28a_Human_H4

42633 Homo sapiens Bacterial Expression

Plasmid Information

Depositor

Joe Landry

Insert

Human H4 (H4C1 Human)

Tags

Untagged

Promoter

T7

Available Since

Feb. 25, 2013

Availability

Available

Sequence Information

Length

967 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	34..52	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

promoter 2

Label	Gene	Product	Position	Strand
T7 promoter	—	—	451..470	Rev
DNA Sequence TAATACGACTCACTATAGG
lacI promoter	lacI	—	778..856	Fwd
DNA Sequence GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	426..451	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC