Pet28a_Human_H3.3

42632 Homo sapiens Bacterial Expression

Plasmid Information

Depositor

Joe Landry

Insert

Human H3.3 (H3-3A Human)

Tags

Untagged

Promoter

T7

Available Since

Feb. 25, 2013

Availability

Available

Sequence Information

Length

1,046 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	34..52	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

promoter 2

Label	Gene	Product	Position	Strand
T7 promoter	—	—	550..569	Rev
DNA Sequence TAATACGACTCACTATAGG
lacI promoter	lacI	—	877..955	Fwd
DNA Sequence GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	525..550	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC