pHisAlix

42576 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
James Hurley
Article
Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10
Insert
ALIX (PDCD6IP Human)
Tags
HIS-TEV
Mutation
silent mutation A1617G (numbering in ALIX gene)
Available Since
Dec. 19, 2013
Availability
Available

Sequence Information

Length
922 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 61..79 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 100..121 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG