pHIS-Hsv2

42524 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
James Hurley
Article
Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:
Insert
Hsv2
Tags
HIS-TEV
Available Since
July 25, 2013
Availability
Available

Sequence Information

Length
947 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 61..79 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 100..121 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG