UMPSA — PlasmidDB

Plasmid Information

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

UMPSA (UMPS Human)

Length

960 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	38..56	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH
6xHis	—	6xHis affinity tag	105..123	Rev
DNA Sequence CACCATCATCACCACCAT Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	821..840	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	796..821	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC