Zebrafish-gRNA-0001

42241 Danio rerio Bacterial Expression

Plasmid Information

Purpose
gRNA targeted to zebrafish gene apoea
Depositor
Keith Joung
Article
Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
Insert
gRNA-apoea (apoea Zebrafish)
Promoter
T7
Available Since
Jan. 31, 2013
Availability
Available

Sequence Information

Length
717 bp
Topology
linear
Molecule Type
genomic DNA

Features

promoter 2
Label Gene Product Position Strand
T7 promoter 237..256 Rev
DNA Sequence
TAATACGACTCACTATAGG
cat promoter 480..583 Fwd
DNA Sequence
TGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAA
terminator 1
Label Gene Product Position Strand
tonB terminator 448..480 Fwd
DNA Sequence
AGTCAAAAGCCTCCGACCGGAGGCTTTTGACT