pET11a-Z-NspGFP

40729 Synthetic Bacterial Expression

Plasmid Information

Depositor
Brian McNaughton
Article
Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub
Insert
N super positive GFP - Leucine Zipper
Tags
His
Promoter
T7
Available Since
Nov. 26, 2012
Availability
Available

Sequence Information

Length
991 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
WELQut site — WELQut protease recognition and cleavag… 59..71 Rev
DNA Sequence
TGGGAGTTACAA
Protein Sequence
WELQ
6xHis — 6xHis affinity tag 623..641 Rev
DNA Sequence
CATCACCACCACCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 716..735 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 691..716 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC