pGEX4T-1_OCT4_T234E_S235E

40635 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
James Thomson
Article
Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109
Insert
POU5F1 (POU5F1 Human)
Tags
GST, TEV Cleavage, and Thrombin Cleavage
Mutation
T234E_S235E
Promoter
tac
Available Since
July 26, 2013
Availability
Available

Sequence Information

Length
443 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 17..35 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCT
Protein Sequence
LVPRGS
TEV site tobacco etch virus (TEV) protease recog… 41..62 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG