pRS-45

40586 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Harald Schwalbe
Article
Silvers et al Protein Expr Purif. 2012 Jul 24;85(1):14
Insert
yTAND12-TEV-hTBPc (TAF1, TBP Human, Budding Yeast)
Tags
His-tag and yTAND12
Promoter
T7
Available Since
Sept. 20, 2012
Availability
Available

Sequence Information

Length
866 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 126..144 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 153..171 Fwd
DNA Sequence
CTGGTGCCGCGTGGTAGC
Protein Sequence
LVPRGS
TEV site — tobacco etch virus (TEV) protease recog… 405..426 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGGGC
Protein Sequence
ENLYFQG
promoter 1
Label Gene Product Position Strand
T7 promoter — — 27..46 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 46..71 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC