pET21b-LOV-ipaA L514K L531E

40253 Synthetic Bacterial Expression

Plasmid Information

Depositor
Brian Kuhlman
Article
Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.
Insert
LOV-ipaA L514K L531E
Tags
6xHis
Mutation
L514K; L531E; Jα helix region hybridized (AsLOV2 …
Promoter
T7
Available Since
Sept. 4, 2012
Availability
Available

Sequence Information

Length
909 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 41..59 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
6xHis — 6xHis affinity tag 539..557 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 623..671 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG