pET21b-LOV-ipaA

40236 Synthetic Bacterial Expression

Plasmid Information

Depositor
Brian Kuhlman
Article
Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.
Insert
LOV-ipaA
Tags
6xHis
Mutation
Jα helix region hybridized (AsLOV2 aa 537-546/ipa…
Promoter
T7
Available Since
Sept. 4, 2012
Availability
Available

Sequence Information

Length
889 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 33..51 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
6xHis — 6xHis affinity tag 531..549 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 615..663 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG