EKAR2G_design2_mTFP_wt_Venus_229

39998 Synthetic Bacterial Expression

Plasmid Information

Depositor
Olivier Pertz
Article
Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi
Insert
Erk activity reporter
Tags
His-Myc
Promoter
CMV
Available Since
Nov. 2, 2012
Availability
Available

Sequence Information

Length
843 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 187..205 Fwd
DNA Sequence
CACCATCACCACCATCAC
Protein Sequence
HHHHHH
Myc — Myc (human c-Myc proto-oncogene) epitop… 214..244 Fwd
DNA Sequence
GAGCAGAAACTAATATCAGAGGAGGATCTT
Protein Sequence
EQKLISEEDL
promoter 1
Label Gene Product Position Strand
p10 promoter — — 57..167 Fwd
DNA Sequence
GACCTTTAATTCAACCCAACACAATATATTATAGTTAAATAAGAATTATTATCAAATCATTTGTATATTAATTAAAATACTATACTGTAAATTACATTTTATTTACAATC
protein_bind 1
Label Gene Product Position Strand
lac operator — — 17..42 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC