EKAR2G_design2_mTFP_wt_Venus_wt

39994 Synthetic Bacterial Expression

Plasmid Information

Depositor
Olivier Pertz
Article
Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi
Insert
Erk activity reporter
Tags
His-Myc
Promoter
CMV
Available Since
July 5, 2013
Availability
Available

Sequence Information

Length
869 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 163..181 Fwd
DNA Sequence
CACCATCACCACCATCAC
Protein Sequence
HHHHHH
Myc — Myc (human c-Myc proto-oncogene) epitop… 190..220 Fwd
DNA Sequence
GAGCAGAAACTAATATCAGAGGAGGATCTT
Protein Sequence
EQKLISEEDL
promoter 1
Label Gene Product Position Strand
p10 promoter — — 33..143 Fwd
DNA Sequence
GACCTTTAATTCAACCCAACACAATATATTATAGTTAAATAAGAATTATTATCAAATCATTTGTATATTAATTAAAATACTATACTGTAAATTACATTTTATTTACAATC