TH

39079 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
TH (TH Human)
Tags
Tev His Flag
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
914 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 24..42 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
FLAG FLAG(R) epitope tag, followed by an ent… 91..115 Rev
DNA Sequence
GATTACAAGGATGACGACGATAAG
Protein Sequence
DYKDDDDK
6xHis 6xHis affinity tag 115..133 Rev
DNA Sequence
CACCATCATCACCACCAT
Protein Sequence
HHHHHH
TEV site tobacco etch virus (TEV) protease recog… 133..154 Rev
DNA Sequence
GAGAACCTCTACTTCCAATCG
Protein Sequence
ENLYFQS