HSP90B1

39076 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
HSP90B1 (HSP90B1 Human)
Tags
TEV-His6-Flag
Available Since
May 7, 2013
Availability
Available

Sequence Information

Length
992 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
TEV site tobacco etch virus (TEV) protease recog… 820..841 Fwd
DNA Sequence
GAGAACCTCTACTTCCAATCG
Protein Sequence
ENLYFQS
6xHis 6xHis affinity tag 841..859 Fwd
DNA Sequence
CACCATCATCACCACCAT
Protein Sequence
HHHHHH
FLAG FLAG(R) epitope tag, followed by an ent… 859..883 Fwd
DNA Sequence
GATTACAAGGATGACGACGATAAG
Protein Sequence
DYKDDDDK
6xHis 6xHis affinity tag 934..952 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH