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Plasmids
BRD2
BRD2
39074
Homo sapiens
Bacterial Expression
Plasmid Information
Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
BRD2 (BRD2 Human)
Tags
His Tev
Available Since
March 18, 2013
Availability
Available
Sequence Information
Length
885 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org
Features
CDS
2
Label
Gene
Product
Position
Strand
TEV site
—
tobacco etch virus (TEV) protease recog…
18..39
Fwd
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
6xHis
—
6xHis affinity tag
443..461
Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator
1
Label
Gene
Product
Position
Strand
T7 terminator
—
—
527..575
Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG